Anti-INO80 antibody (ab105451)

Overview

  • Product nameAnti-INO80 antibody
    See all INO80 primary antibodies
  • Description
    Rabbit polyclonal to INO80
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human INO80.

  • Positive control
    • This antibody gave a positive signal in Human Brain tissue lysate and HeLa whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionDNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.
  • Tissue specificityAccording to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 DBINO domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe DBINO region is involved in binding to DNA.
  • Cellular localizationNucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA helicase INO80 antibody
    • hINO80 antibody
    • homolog of yeast INO80 antibody
    • INO80 antibody
    • INO80 complex subunit A antibody
    • INO80 homolog antibody
    • INO80_HUMAN antibody
    • INO80A antibody
    • INOC1 antibody
    • KIAA1259 antibody
    • Putative DNA helicase INO80 complex homolog 1 antibody
    see all

Anti-INO80 antibody images

  • All lanes : Anti-INO80 antibody (ab105451) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 177 kDa
    Observed band size : 177 kDa
    Additional bands at : 32 kDa,64 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab105451 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105451, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2, MCF7 cells at 1µg/ml.

References for Anti-INO80 antibody (ab105451)

ab105451 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Based on the sequence homology with the immunogen used to generate this antibody there is low likelihood of reactivity with S.cerevisiae.

I hope this information is helpful to you. Please do not hesitate to contact...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse embryo OCT section e16.5)
Specification Mouse embryo OCT section e16.5
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Mr. Andrew McKay

Verified customer

Submitted Oct 25 2012

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product qu...

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I am very pleased to hear you would like to accept our offer and test ab105451 in mouse. This code will give you a discount off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include...

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Thank you very much for your interest in ab105451. We have tested and would guarantee this antibody to work in WB and ICC/IF using human samples.

Would your KO be in mouse? We don't have sample sizes of our products because we carry so many a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"