Anti-INO80 antibody (ab118787)
Key features and details
- Rabbit polyclonal to INO80
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80 antibody
See all INO80 primary antibodies -
Description
Rabbit polyclonal to INO80 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat -
Immunogen
Synthetic peptide corresponding to a region within amino acids 1506-1556 of Human INO80 (NP_060023.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 176 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 176 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle. -
Tissue specificity
According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 DBINO domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
The DBINO region is involved in binding to DNA. -
Cellular localization
Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 478262 Dog
- Entrez Gene: 54617 Human
- Entrez Gene: 100517567 Pig
- Entrez Gene: 100350680 Rabbit
- Omim: 610169 Human
- SwissProt: Q9ULG1 Human
- Unigene: 292949 Human
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Alternative names
- DNA helicase INO80 antibody
- hINO80 antibody
- homolog of yeast INO80 antibody
see all
Images
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All lanes : Anti-INO80 antibody (ab118787) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 176 kDa
Exposure time: 30 seconds -
Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab118787 has been referenced in 12 publications.
- Cheon Y et al. The chromatin remodeler Ino80 mediates RNAPII pausing site determination. Genome Biol 22:294 (2021). PubMed: 34663418
- Ka NL et al. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Cell Rep 37:110138 (2021). PubMed: 34936865
- Prendergast L et al. Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability. Nat Commun 11:4534 (2020). PubMed: 32913330
- Moreno-Andrés D et al. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 9:N/A (2020). PubMed: 32708675
- Schutt C et al. Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy. EMBO J 39:e105098 (2020). PubMed: 32960481