Anti-INO80C antibody (ab151046)
Key features and details
- Rabbit polyclonal to INO80C
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80C antibody -
Description
Rabbit polyclonal to INO80C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment, corresponding to a sequence from Human INO80C.
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Positive control
- ICC: U-2 OS whole cells; IHC-P: Human kidney, testits and skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Relevance
Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 125476 Human
- Entrez Gene: 291737 Rat
- SwissProt: Q6PI98 Human
- SwissProt: Q5BJY3 Rat
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Alternative names
- C18orf37 antibody
- FLJ38183 antibody
- hIes6 antibody
see all
Images
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Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling INO80C at the nucleoli fibrilar center with ab151046 at 2 µg/ml.
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Immunohistochemistry analysis of human kidney tissue labeling INO80C of tubules cells with ab151046 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry analysis of human testis tissue labeling INO80C at the nuclues of seminiferous ducts with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry analysis of human skeletal muscle tissue labeling INO80C of myocytes with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab151046 staining INO80C in Mouse paraffin-fixed tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in Goat serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal (1/125) was used as the secondary antibody. Red - INO80C, Blue (DAPI) - nuclei.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151046 has not yet been referenced specifically in any publications.