Anti-iNOS antibody (ab15323)
Key features and details
- Rabbit polyclonal to iNOS
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-iNOS antibody
See all iNOS primary antibodies -
Description
Rabbit polyclonal to iNOS -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 84% homology with Rat iNOS. Ab15323 has been batch tested using mouse lysates/tissues only. Some customers have successfully used ab15323 with rat. Please contact Abcam Scientific Support for more information.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse iNOS.
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Positive control
- IHC-P: FFPE mouse and rat lung tissue sections.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (10) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 131 kDa).
Please use at a assay dependent concentration. 1µg/ml was used of a 0.2mg/ml stock (lot GR126616-1). Otherwise 1/250 is recommended for any batches at lower concentration. |
Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 131 kDa). Please use at a assay dependent concentration. 1µg/ml was used of a 0.2mg/ml stock (lot GR126616-1). Otherwise 1/250 is recommended for any batches at lower concentration. |
Target
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Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
Tissue specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 4843 Human
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- Omim: 163730 Human
- SwissProt: P35228 Human
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 709191 Human
see all -
Alternative names
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
Images
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All lanes : Anti-iNOS antibody (ab15323) at 1 µg/ml
Lane 1 : Raw264.7
Lane 2 : Raw264.7 + LPS (1 ug per mL; 18 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR- goat anti-rabbit (green) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDaThis blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with rabbit polyclonal to iNOS (ab15323; 1 ug x mL-1 (based on antibody concentration of 0.2mg/ml - lot GR126616-1) and the loading control mouse anti-GAPDH antibody (ab8245; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green; 1:10000) and IR-goat anti-mouse (red; 1:10000) for 1 hour at room temperature before imaging.
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All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml
Lane 1 : Raw 264.7 whole cell lysate + PMA
Lane 2 : Raw 264.7 whole cell lysate + PMA + LPS + Brefeldin A
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
All lanes : Anti-iNOS antibody (ab15323) at 5 µg/ml
Lane 1 : Human recombinant iNOS protein
Lane 2 : Mouse recombinant iNOS protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Exposure time: 1 minuteMembrane was blocked in 2% milk for 1 hour at RT. Primary anitbody was incubated at 4°C overnight.
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IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (526)
ab15323 has been referenced in 526 publications.
- Azouz RA et al. Silica Nanoparticle-Induced Reproductive Toxicity in Male Albino Rats via Testicular Apoptosis and Oxidative Stress. Biol Trace Elem Res 201:1816-1824 (2023). PubMed: 35672543
- El-Kashef DH & Sharawy MH Hepatoprotective effect of nicorandil against acetaminophen-induced oxidative stress and hepatotoxicity in mice via modulating NO synthesis. Environ Sci Pollut Res Int 30:14253-14264 (2023). PubMed: 36149558
- Liu S et al. Immunomodulatory hybrid micro-nanofiber scaffolds enhance vascular regeneration. Bioact Mater 21:464-482 (2023). PubMed: 36185748
- Missiroli S et al. PML at mitochondria-associated membranes governs a trimeric complex with NLRP3 and P2X7R that modulates the tumor immune microenvironment. Cell Death Differ 30:429-441 (2023). PubMed: 36450825
- Osonoi S et al. RAGE activation in macrophages and development of experimental diabetic polyneuropathy. JCI Insight 7:N/A (2022). PubMed: 36477360