Overview

  • Product nameAnti-iNOS antibody
    See all iNOS primary antibodies
  • Description
    Rabbit polyclonal to iNOS
  • SpecificityThis antibody detects endogenous levels of total iNOS protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human iNOS. Immunogen is in the range of aa 1101-1150
    Database link: P35228

  • Positive control
    • Human lung carcinoma tissue and extracts from NIH/3T3 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificityExpressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Anti-iNOS antibody images

  • All lanes : Anti-iNOS antibody (ab53769) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells, plus peptide


    Predicted band size : 131 kDa
    Observed band size : 131 kDa
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53769 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with peptide.

References for Anti-iNOS antibody (ab53769)

This product has been referenced in:
  • McAdam E  et al. Inducible Nitric Oxide Synthase (iNOS) and Nitric Oxide (NO) are Important Mediators of Reflux-induced Cell Signalling in Esophageal Cells. Carcinogenesis : (2012). IHC . Read more (PubMed: 22826608) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 24 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Dec 12 2014

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.
 
Ich habe die Homologie der Immunogene unsere Anti iNOS Antikörper mit Schwein verglichen und habe folgende mögliche Antikörper für Sie:
ab129372 : 85%
ab13691...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"