Overview

  • Product name
    Anti-iNOS antibody [NOS-IN]
    See all iNOS primary antibodies
  • Description
    Mouse monoclonal [NOS-IN] to iNOS
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    FSYGAKKGSALEEPKATRL

    conjugated to KLH, corresponding to amino acids 1126-1144 of Mouse iNOS

  • Positive control
    • Whole cell extract of the murine macrophage cell line RAW 264.7 activated with LPS and IFN-gamma
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab15323 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab49999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000. Predicted molecular weight: 131 kDa.

Target

  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • Tissue specificity
    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence  analysis of IFN-γ/LPS-activated RAW-264.7 cells labeling iNOS  with ab49999 at 1/2000 dilution. Cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat Anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Lane 1 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution
    Lane 2 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution
    Lane 3 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution
    Lane 4 : Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution

    All lanes : IFN-gamma/LPS-activated RAW-264.7 cell extract

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate

    Predicted band size: 131 kDa

References

This product has been referenced in:
  • Lam D  et al. Responses of rat and mouse primary microglia to pro- and anti-inflammatory stimuli: molecular profiles, K+channels and migration. J Neuroinflammation 14:166 (2017). WB ; Rat . Read more (PubMed: 28830445) »
  • Messeha SS  et al. The attenuating effects of plumbagin on pro-inflammatory cytokine expression in LPS-activated BV-2 microglial cells. J Neuroimmunol 313:129-137 (2017). Mouse . Read more (PubMed: 28950995) »

See all 16 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Rat Cell lysate - whole cell (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 27 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2014

Thank you for your interest.

The immunogen used to raise ab49999 was a synthetic peptide: FSYGAKKGSALEEPKATRL conjugated to KLH, corresponding to amino acids 1126-1144 of Mouse iNOS.

According to blast search the aa homology between...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (GTN, Skeletal muscle)
Loading amount
50 µg
Specification
GTN, Skeletal muscle
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted Jul 05 2012

Thank you for your note.
This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab49999 (exchange for the original product ab15323) as a free of charge replacement. I hope the second via...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebral sections)
Specification
Cerebral sections
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
glycine as blocking agent for 15 minute(s) · Concentration: 1%
Username

Charlotte

Verified customer

Submitted Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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