Inosine Quantification Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.
Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.
|Converter Enzyme Mix (Lyophilized)||Green||1 vial|
|Developer Enzyme Mix (Lyophilized)||Brown||1 vial|
|Inosine Assay Buffer||WM||1 x 25ml|
|Inosine Standard (10 mM)||Yellow||1 vial|
|Inosine Substrate Mix (Lyophilized)||Red||1 vial|
|PicoProbe||Blue||1 x 0.4ml|
Our Abpromise guarantee covers the use of ab126286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab126286 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"