Inosine Quantification Assay Kit (Fluorometric) (ab126286)

Overview

  • Product name
    Inosine Quantification Assay Kit (Fluorometric)
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    0.1 nmol/well
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammal
  • Product overview

    Inosine Quantification Assay Kit (Fluorometric) (ab126286) is a kit where inosine is converted to hypoxanthine, which then will react with the substrate mix and PicoProbe to generate fluorescence (Ex/Em = 535/587 nm) in presence of converter and developer enzymes. This assay has a detection limit of of approximately 100 pmol inosine/well.

  • Notes

    Inosine is commonly found in tRNAs and is essential for proper translation of the genetic code in wobble base pairs. Knowledge of inosine metabolism has led to advances in immunotherapy in recent decades. Inosine and hypoxanthine are also potential markers of ATP catabolic-by-products from oxidative stress which follows acute cardiac ischemia.

     

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab126286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Inosine standard curve generated using this kit protocol.

Protocols

References

ab126286 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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