Overview

  • Product nameAnti-INPP4B antibody [EPR3108Y]
    See all INPP4B primary antibodies
  • Description
    Rabbit monoclonal [EPR3108Y] to INPP4B
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human INPP4B aa 1-100 (N terminal).
    Database link: O15327

  • Positive control
    • MCF7, fetal skeletal muscle, fetal heart lysates and muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3108Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
IHC-P 1/50.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionCatalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate.
    • Tissue specificityWidely expressed with highest levels occurring in the skeletal muscle and heart.
    • PathwaySignal transduction; phosphatidylinositol signaling pathway.
    • Sequence similaritiesBelongs to the inositol-3,4-bisphosphate 4-phosphatase family.
      Contains 1 C2 domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Inositol polyphosphate 4 phosphatase, type II antibody
      • 4-bisphosphate 4-phosphatase antibody
      • Inositol polyphosphate 4 phosphatase type II 105kDa antibody
      • Inositol polyphosphate 4-phosphatase type II antibody
      • INP4B_HUMAN antibody
      • Inpp4b antibody
      • MGC132014 antibody
      • Type II inositol 3,4 bisphosphate 4 phosphatase antibody
      • Type II inositol-3 antibody
      see all

    Anti-INPP4B antibody [EPR3108Y] images

    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

      INPP4B positive expression shows strong cytoplasmic staining. Magnification: X40.

    • All lanes : Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/5000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Fetal skeletal muscle lysate
      Lane 3 : Fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 107 kDa
      Observed band size : 107 kDa
    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human squamous cell carcinoma tissue samples sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with ab81269 (1/50) for 25 minutes at room temperature.

      INPP4B negative expression. Magnification: X40.

    • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human skeletal muscle tissue (negative control) sectioned at 4 μm and placed at 60°C for 1 hour. Slides were deparaffinized and antigen retrieval was carried out by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. Samples were incubated with primary antibody substituted for antibody diluent for 25 minutes at room temperature.

      Magnification: X40.

    • Immunohistochemical analysis of paraffin-embedded muscle tissue using ab81269 at 1/50 dilution.

    References for Anti-INPP4B antibody [EPR3108Y] (ab81269)

    This product has been referenced in:
    • Asleh-Aburaya K  et al. Basal biomarkers nestin and INPP4b identify intrinsic subtypes accurately in breast cancers that are weakly positive for oestrogen receptor. Histopathology N/A:N/A (2016). Read more (PubMed: 27402148) »
    • Sakr RA  et al. PI3K Pathway Activation in High-Grade Ductal Carcinoma In Situ--Implications for Progression to Invasive Breast Carcinoma. Clin Cancer Res N/A:N/A (2014). IHC-P . Read more (PubMed: 24634376) »

    See all 4 Publications for this product

    Product Wall

    Thank you for contacting us and your interest in our products.

    The immunogen sequence used to raise the anti-INPP4B antibody (ab81269) is proprietary information. However, in order to aid your research I am able to share it with you. Please ...

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    Thank you for contacting us. My colleague Claudia is currently out of the office. We have been informed from the source of ab81269 that they still currently have over 4mL of lot GR34190 in stock. It is hard to give you an estimation of the date as t...

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    Thank you very much for your feedback. I am very happy to hear that the new vial allowed good results. We do currently have one other vial of GR34190-2 in stock and a few vials of GR34190-3. So please do specify you would like to have the GR34190-2. As...

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    Thank you very much for having sent all this additional information. I am very sorry to see that there is a problem with one of these vials. I will send this information also to the laboratory and hope that we can restest the new batch. Thank you very ...

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    Thank you for calling us. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am sorry to hear that there seems to be a problem with the new batch of this antibody. As we disc...

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    Thank you for contacting us with your question. The concentration of ab81269 lot 863038 is 0.038 mg/mL, and the recommended starting dilution for IHC is 1:50. I hope this information is helpful, but please let me know if you have any further que...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human breast cell lines)
    Loading amount 10 µg
    Specification Human breast cell lines
    Gel Running Conditions Reduced Denaturing (4-12% NuPAGE)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Damian Yap

    Verified customer

    Submitted Apr 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"