Overview

  • Product nameAnti-INPP5F antibody
    See all INPP5F primary antibodies
  • Description
    Rabbit polyclonal to INPP5F
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 591-857 of the Human INPP5F protein (Q01968).

  • Positive control
    • 293T and A431 cell lysates, A431 cells and Human Breast ca tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab125917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 104 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM
ICC/IF 1/100 - 1/500.

Target

  • FunctionConverts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.
  • Tissue specificityBrain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts.
  • Involvement in diseaseDefects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
    Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones.
  • Sequence similaritiesBelongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
    Contains 1 Rho-GAP domain.
  • Cellular localizationEndosome. Also found on macropinosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.1.3.36 antibody
    • Inositol polyphosphate 5 phosphatase OCRL 1 antibody
    • Inositol polyphosphate 5 phosphatase OCRL1 antibody
    • Inositol polyphosphate 5-phosphatase OCRL-1 antibody
    • INPP5F antibody
    • LOCR antibody
    • Lowe oculocerebrorenal syndrome protein antibody
    • NPHL2 antibody
    • OCRL 1 antibody
    • OCRL antibody
    • OCRL_HUMAN antibody
    • OCRL1 antibody
    • Oculocerebrorenal syndrome of Lowe antibody
    • Phosphatidylinositol polyphosphate 5 phosphatase antibody
    see all

Anti-INPP5F antibody images

  • All lanes : Anti-INPP5F antibody (ab125917) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.
  • ab125917, at 1/500, staining INPP5F in Human A431 cells (methanol-fixed) by immunofluorescence (panel 1) and co-stained with Hoechst 33342 (panel 2).
  • ab125917, at 1/250, staining INPP5F in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

References for Anti-INPP5F antibody (ab125917)

ab125917 has not yet been referenced specifically in any publications.

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