KLH conjugated synthetic peptide corresponding to a region between amino acids 416-446 from the central region of Human INSC (UniProt ID: Q1MX18).
Our Abpromise guarantee covers the use of ab102953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 63 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 5 µg/ml.|
ICC/IF image of ab102953 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102953 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab102953 has not yet been referenced specifically in any publications.