Anti-INSC antibody (ab102953)


  • Product nameAnti-INSC antibody
    See all INSC primary antibodies
  • Description
    Rabbit polyclonal to INSC
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region between amino acids 416-446 from the central region of Human INSC (UniProt ID: Q1MX18).

  • Positive control
    • WiDr cell line lysate; Hepatocarcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.



Our Abpromise guarantee covers the use of ab102953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionMay function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium.
  • Tissue specificityIsoform 1 is expressed in various tissues with stronger expression in liver, kidney and small intestine. Isoform 2 is abundantly expressed in small intestine and to a lower extent in lung and pancreas.
  • Developmental stageExpressed in fetal cochlea.
  • Cellular localizationCytoplasm. Localizes to the apical cortex of mitotic epidermal cells and to the apical side of neuronal progenitor cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Insc antibody
    • INSC_HUMAN antibody
    • Inscuteable homolog (Drosophila) antibody
    • Protein inscuteable homolog antibody

Anti-INSC antibody images

  • ICC/IF image of ab102953 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102953 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-INSC antibody (ab102953) at 1/100 dilution + WiDr cell line lysate at 35 µg

    Predicted band size : 63 kDa
  • Immunohistochemical analysis of INSC in formalin fixed and paraffin embedded hepatocarcinoma tissue using ab102953 at a 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-INSC antibody (ab102953)

ab102953 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse Embryo (day 8.5))
Specification Mouse Embryo (day 8.5)
Fixative Paraformaldehyde
Permeabilization Yes - Saponin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Derek Passer

Verified customer

Submitted Oct 27 2011

Thanks for your call today and your interest in our INSC antibodies. DISCOUNT CODES: ab102953: *** ab107096: *** Expiration Date: February 14, 2012 Since these antibodies have not been tested in IHC-Fr on ...

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