Overview

  • Product name
  • Description
    Rabbit polyclonal to Insig1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 129 - 178 (TWLFHKTRSN YRVFLKSPIV IESSKPPILR ARKILEENLT VDYDKDYLFS) of human INSIG1 (NP_938150).

  • Positive control
    • Human fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
  • Tissue specificity
    Expressed in all tissues tested with highest expression in the liver.
  • Sequence similarities
    Belongs to the INSIG family.
  • Post-translational
    modifications
    Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CL 6 antibody
    • CL6 antibody
    • INSI1_HUMAN antibody
    • INSIG 1 antibody
    • INSIG 1 membrane protein antibody
    • INSIG-1 antibody
    • insig1 antibody
    • INSIG1 membrane protein antibody
    • Insulin induced gene 1 antibody
    • Insulin induced gene 1 protein antibody
    • Insulin-induced gene 1 protein antibody
    • MGC1405 antibody
    see all

Images

  • Anti-Insig1 antibody (ab86350) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References

ab86350 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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