Anti-Insig1 antibody - N-terminal (ab175856)

Overview

  • Product name
    Anti-Insig1 antibody - N-terminal
    See all Insig1 primary antibodies
  • Description
    Rabbit polyclonal to Insig1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Insig1 aa 42-72 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O15503

  • Positive control
    • Hela cell line lysate; Human prostate carcinoma tissue; Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 30 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
  • Tissue specificity
    Expressed in all tissues tested with highest expression in the liver.
  • Sequence similarities
    Belongs to the INSIG family.
  • Post-translational
    modifications
    Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CL 6 antibody
    • CL6 antibody
    • INSI1_HUMAN antibody
    • INSIG 1 antibody
    • INSIG 1 membrane protein antibody
    • INSIG-1 antibody
    • insig1 antibody
    • INSIG1 membrane protein antibody
    • Insulin induced gene 1 antibody
    • Insulin induced gene 1 protein antibody
    • Insulin-induced gene 1 protein antibody
    • MGC1405 antibody
    see all

Images

  • Anti-Insig1 antibody - N-terminal (ab175856) at 1/1000 dilution + Hela cell line lysate at 35 µg

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Insig1 with ab175856 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HeLa cells labeling Insig1 with ab175856 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175856 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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