The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 30 kDa.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
Expressed in all tissues tested with highest expression in the liver.
Belongs to the INSIG family.
Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Insig1 with ab175856 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HeLa cells labeling Insig1 with ab175856 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.