Overview

  • Product nameAnti-Insig2 antibody
    See all Insig2 primary antibodies
  • Description
    Rabbit polyclonal to Insig2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (AEGETESPGP KKCGPYISSV TSQSVNLMIR GVVLFFIGVF LALVLNLLQI) of human INSIG2 (NP_057217).

  • Positive control
    • Human fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceInsig2 is highly similar to the protein product encoded by gene Insig1. Both Insig1 protein and Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • Hypothetical protein INSIG2 antibody
    • INSIG 2 antibody
    • INSIG 2 membrane protein antibody
    • INSIG2 membrane protein antibody
    • Insulin induced gene 2 antibody
    • Insulin induced gene 2 protein antibody
    • Insulin induced protein 2 antibody
    • MGC26273 antibody
    see all

Anti-Insig2 antibody images

  • Anti-Insig2 antibody (ab86351) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 28 kDa,<22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Insig2 antibody (ab86351)

ab86351 has not yet been referenced specifically in any publications.

Product Wall

DISCOUNT CODE: ***
Expiration date: September 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125186 using mouse or rat samplesand include this cod...

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Thank you for your reply and for the additional information.

I am sending a vial of ab86415 free of charge on the order ***. This antibody is in stock and should arrive on Monday. Please keep me updated about how this antibody works in your W...

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Thank you for contacting us and for letting us know about the trouble with ab86351. I am sorry to see that the results have been so poor.

I would be happy to send a vial of ab86415 as a replacement. Do you have your original order or PO numbe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"