Overview

  • Product name
  • Description
    Rabbit polyclonal to INSM1
  • Specificity
    Ab30940 recognises INSM1.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide derived from residues 393 - 442 of human INSM1.

    .

  • Positive control
    • Fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21609490Cells fixed with 4% PFA, treated with 0.5% Triton for 5 minutes at Room Temperature.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/7.8125e+006.

Target

  • Relevance
    INSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • IA1 antibody
    • Insulinoma associated protein 1 antibody
    • Zinc finger protein IA1 antibody

Images

  • Anti-INSM1 antibody (ab30940) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

References

This product has been referenced in:
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). ICC/IF, WB . Read more (PubMed: 21609490) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain 2 day-old mouse)
Loading amount
20 µg
Specification
Brain 2 day-old mouse
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

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Verified customer

Submitted Jun 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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