The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21609490Cells fixed with 4% PFA, treated with 0.5% Triton for 5 minutes at Room Temperature.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceINSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
Anti-INSM1 antibody (ab30940) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg/ml
Secondary HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size : 53 kDa Observed band size : 53 kDa
References for Anti-INSM1 antibody (ab30940)
This product has been referenced in:
Kamakura M et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J8:257 (2011).
Read more (PubMed: 21609490) »