Anti-Insulin + Proinsulin antibody [D3E7] (ab8305)
Key features and details
- Mouse monoclonal [D3E7] to Insulin + Proinsulin
- Suitable for: ELISA, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Insulin + Proinsulin antibody [D3E7]
See all Insulin + Proinsulin primary antibodies -
Description
Mouse monoclonal [D3E7] to Insulin + Proinsulin -
Host species
Mouse -
Specificity
Kd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin -
Tested applications
Suitable for: ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig -
Immunogen
Full length native protein (purified). Native human insulin
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General notes
Detection of insulin and proinsulin
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
Detection of insulin and proinsulin -
Clonality
Monoclonal -
Clone number
D3E7 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Relevance
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 280829 Cow
- Entrez Gene: 3630 Human
- Entrez Gene: 16333 Mouse
- Entrez Gene: 16334 Mouse
- Entrez Gene: 24505 Rat
- Omim: 176730 Human
- SwissProt: P01317 Cow
- SwissProt: P01308 Human
see all -
Alternative names
- ILPR antibody
- ins antibody
- Insulin antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab8305 has been referenced in 1 publication.
- Criscimanna A et al. Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice. Gastroenterology 141:1451-62, 1462.e1-6 (2011). IHC-Fr ; Mouse . PubMed: 21763240