Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756)

Abreviews (4)Q&A (6)Specific References (2)

Overview

  • Product name
    Anti-Insulin + Proinsulin antibody [D3E7] (Biotin)
    See all Insulin + Proinsulin primary antibodies
  • Description
    Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: IHC-Fr, Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Full length protein (Human).

  • General notes
    For concentration (OD280nm, E0.1% = 1.4). Affinity constant Kd = 6.3 x 10E-8M.

Properties

Applications

Our Abpromise guarantee covers the use of ab20756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Can be paired for sandwich ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).

Target

Images

  • Immunohistochemistry (Frozen sections) - Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756)
    Immunohistochemistry (Frozen sections) - Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756)This image is courtesy of an anonymous Abreview

    ab20756 staining Insulin + Proinsulin in human pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C followed by incubation with Cy3®-conjugated streptavidin for 30 minutes in the dark at room temperature. Nuclei were counterstained with DAPI.

    See Abreview

References

This product has been referenced in:
  • Kuhn C  et al. Human CD3 transgenic mice: preclinical testing of antibodies promoting immune tolerance. Sci Transl Med 3:68ra10 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21289272) »
  • Yang RY  et al. Ablation of a galectin preferentially expressed in adipocytes increases lipolysis, reduces adiposity, and improves insulin sensitivity in mice. Proc Natl Acad Sci U S A 108:18696-701 (2011). ELISA ; Mouse . Read more (PubMed: 21969596) »

See all 2 Publications for this product

Publishing research using ab20756? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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I have purchased the Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756) from your esteemed company and I am wondering if you can advise me about the best secondary antibody to be used.

This antibody is already conjugated to biotin and therefore does not need a secondary antibody for detection.
What you will need is avidin or streptavidin conjugated to an enzyme or fluorochrome that will bind to multiple sites on the biotinylated...

Read More

ab100578 chez le rat?



Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même...

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Have you tested your human insulin assay against the insulin analogs, such as humalog (lispro), novolog (aspart), lantus (glarginine). If so what is the cross-reactivity to these analogs.

Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
http://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
http:/...

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Dear Technical

I bought insulin antibody from your company and used this one for sandwich ELISA.

Is it possible to use this antibody for Elispot. Maybe insulin producing cells such as pancreas islets or

insulin secretin...

Read More

Thank you for your further enquiry.

I am sorry to confirm that as far as we are aware, ab8304 and ab20756 insulin antibodies have never been tested in ELISPOT. All tested and guaranteed applications are specified on our datasheets, and these...

Read More

Hello

I will purchase anti-insulin antibodies (ab8304, ab20756) from your company.

Maybe I will use ab8304as a coating antibody and ab20756 as a detection antibody. Both are monoclonal antibody from mouse.

These monoclo...

Read More

Thank you for your enquiry which has been forwarded to the scientific support team. We can be contacted at mailto:technical@abcam.com, or directly from the website.

I can confirm that ab8304 can be paired for Sandwich ELISA with Mouse monocl...

Read More

I will purchase anti-insulin antibodies (ab8304, ab20756) from your company.
Maybe I will use ab8304as a coating antibody and ab20756 as a detection antibody. Both are monoclonal antibody from mouse.
These monoclonal antibodies were from mous...

Read More

Thank you for contacting us. Yes, the clones D6C4 and D3E7 have been validated for use as a sandwich ELISA pair and will be guaranteed for that purpose. Because the detection antibody you are choosing is already biotinylated, it will not be an issue th...

Read More

Immunohistochemistry (Frozen sections) abreview for Anti-Insulin + Proinsulin antibody [D3E7] (Biotin)

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Read More
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Username

Abcam user community

Verified customer

Submitted Mar 15 2011

Immunohistochemistry (Frozen sections) abreview for Anti-Insulin + Proinsulin antibody [D3E7] (Biotin)

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Titox x100
Blocking step
Rouche Blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Nov 03 2008

Immunohistochemistry (Frozen sections) abreview for Anti-Insulin + Proinsulin antibody [D3E7] (Biotin)

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Pancreas)
Permeabilization
Yes - 0.25% Triton x100
Specification
Pancreas
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Oct 21 2008

Immunohistochemistry (Frozen sections) abreview for Anti-Insulin + Proinsulin antibody [D3E7] (Biotin)

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3%
Read More
Username

Dr. Anahi Chavarria

Verified customer

Submitted Sep 15 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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