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Kd for this antibody is 8.1 x 10-8M.
Detection of both insulin and proinsulin.
Our Abpromise guarantee covers the use of ab8304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 19628478|
|ELISA||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24614166|
|Sandwich ELISA||Use a concentration of 10 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin) (ab20756) and Mouse monoclonal [D3E7] to Insulin + Proinsulin (HRP) (ab28063). Use at a concentration of 10 µg/ml, 50µL per well. Can be used as Capture antibody with recommended pair(s).|
|IHC-P||1/1250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab8304 at 1/1250 staining developing mouse pancreas tissue sections (E14/15) by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was then blocked and incubated with the antibody for 2 hours. A biotinylated goat anti-mouse IgG antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"