Overview

  • Product name
  • Description
    Rabbit polyclonal to Insulin
  • Specificity
    The antibody may be used to detect B-cells in normal pancreas and in pancreatic tumours.
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
    Predicted to work with: Mammal
  • Immunogen

    Purified porcine Insulin

  • Positive control
    • Pancreas
  • General notes
    Diluted antiserum in PBS pH 7.3 containing BSA and sodium azide (0.09%) as a preservative

Properties

Applications

Our Abpromise guarantee covers the use of ab46716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
  • Application notes
    IHC-Fr: 1/10 - 1/25.
    IHC-P: 1/10 - 1/25.

    Incubation time: 60 min at RT or 2-8°C overnight

    Pre-treatment of the tissue with protease is not required; unmasking of paraffin sections with citrate buffer or unmasking fluid G may increase the staining intensity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
    • Involvement in disease
      Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
      Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
      Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities
      Belongs to the insulin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    References

    ab46716 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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