Our Abpromise guarantee covers the use of ab7842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 24336520|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20335226|
|ICC/IF||1/50 - 1/100.|
|IHC-P||1/50 - 1/100. Prolonged fixation in buffered formalin can destroy the epitope.|
|IHC-Fr||1/50 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21829502|
The picture shows beta cells in the pancreas using ab7842 (primary) and Goat Anti-Guinea pig IgG H&L (Texas Red ®) (ab6906) (secondary). The beta cells in the islets show up very bright red with almost no background.
This image was kindly supplied as part of the review submitted by Kristi Hultman.
ab7842 at 1/200 staining mouse pancreas tissue sections by IHC-P.
The tissue section was prepared by Bouin's fixation followed by 1N HCl antigen retrieval and blocked with 1% BSA, 5% goat serum for 4 hours at 20°C.
The primary antibody was incubated for 16 hours at 4°C.
The secondary antibody was a fluorophore labelled Goat anti-Guinea pig polyclonal diluted 1/200.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"