Overview

  • Product nameAnti-Insulin antibody
    See all Insulin primary antibodies
  • Description
    Guinea pig polyclonal to Insulin
  • SpecificityThis antibody stains beta (b) cells of the Langerhan's islets in human pancreas.
  • Tested applicationsSuitable for: IP, IHC-FoFr, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Syrian Hamster, Other
  • Immunogen

    Insulin (Human).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab7842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 24336520
IHC-FoFr Use at an assay dependent concentration. PubMed: 20335226
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Prolonged fixation in buffered formalin can destroy the epitope.
IHC-Fr 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 21829502

Target

  • FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody images

  • ab7842 staining Insulin in human kidney tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in formaldehyde, permeabilized with methanol , blocked using 3% BSA for 1 hour at 22°C then incubated with ab7842 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an Aleza-Fluor 568 conjugated goat anti-guinea pig polyclonal used at a 1/500 dilution.

    Image shows kidney section bearing RGD-Adv-hHGF-hXIAP-transduced human islets at 30 days after islet transplantation. (a-b) Insulin was stained in red to indicate the functional human islets of mice receiving untransduced human islets (a) and RGD-Adv-hHGF-hXIAP-transduced human islets (b).

    See Abreview

  • The picture shows beta cells in the pancreas using ab7842 (primary) and ab6906 with Texas Red (secondary). The beta cells in the islets show up very bright red with almost no background.

     

    This image was kindly supplied as part of the review submitted by Kristi Hultman. 

     

  • ab7842 at 1/1000 dilution staining rat pancreas tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were fixed with paraformaldehyde and blocked with 5% serum prior to incubation with the antibody for 12 hours. A Cy2 conjuagted goat polyclonal antibody was used as the secondary. Insulin staining is shown in green. Somatostatin staining is shown in red. DAPI was used as nuclear counterstain.

    See Abreview

  • ab7842 at 1/200 staining mouse pancreas tissue sections by IHC-P.

    The tissue section was prepared by Bouin's fixation followed by 1N HCl antigen retrieval and blocked with 1% BSA, 5% goat serum for 4 hours at 20°C.

    The primary antibody was incubated for 16 hours at 4°C.

    The secondary antibody was a fluorophore labelled Goat anti-Guinea pig polyclonal diluted 1/200.

    See Abreview

  • Flow cytometry analysis of a single cell suspension from isolated Human fetal islets, staining Insulin with ab7842.

References for Anti-Insulin antibody (ab7842)

This product has been referenced in:
  • Singhal G  et al. Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas. PLoS One 11:e0148252 (2016). Mouse . Read more (PubMed: 26872145) »
  • Rakshit K  et al. Circadian variation of the pancreatic islet transcriptome. Physiol Genomics 48:677-87 (2016). Read more (PubMed: 27495157) »

See all 84 Publications for this product

Product Wall

Thank you for your patience.

ab7842 has not been tested in the lab in ICC/IF. This application was added to our datasheet based on publications:

Zhu M et al. Lymphotoxin receptor is required for the migration and selection of autor...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization No
Specification Pancreas
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Dec 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM tri-sodium citrate buffer, pH 6.0
Permeabilization No
Specification pancreas
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization Yes - 0.1% Triton X-100
Specification pancreas
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 15 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Whole pancreas)
Permeabilization No
Specification Whole pancreas
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative Formaldehyde
Username

Dr. Susan Sheng

Verified customer

Submitted Feb 12 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Srividya Vasu

Verified customer

Submitted Oct 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Vector Antigen Unmasking Solution
Permeabilization No
Blocking step Peroxidase as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Pancreas)
Specification Pancreas
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Mr. Thomas Schöppe

Verified customer

Submitted Aug 15 2012

Gracias por contactarnos.


Lamentablemente, ab7842 es un anticuerpo no purificado, suministrado como antisuero. Solo podemos proporcionar valores de concentración para anticuerpos purificados ya que las preparaciones de anticuerpo ...

Read More

1-10 of 55 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"