Recombinant
RabMAb

Anti-Insulin antibody [EPR3075] (ab108326)

Overview

  • Product name
    Anti-Insulin antibody [EPR3075]
    See all Insulin primary antibodies
  • Description
    Rabbit monoclonal [EPR3075] to Insulin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Insulin aa 50 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • Human fetal pancreas lysate; Human pancreas tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
    • Involvement in disease
      Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
      Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
      Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities
      Belongs to the insulin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    Images

    • Anti-Insulin antibody [EPR3075] (ab108326) at 1/1000 dilution + Human fetal pancreas lysate at 10 µg

      Predicted band size : 12 kDa
    • ab108326 at 1/100 dilution staining insulin in Human pancreas by Immunohistochemistry, Paraffin-embedded tissue.

    References

    ab108326 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Gracias por escribirnos.

    Si lo que quiere la clienta es un anticuerpo contra C peptide para ICC que reaccione en humano, existen varias opciones.

    Si lo que requiere además es que no reaccione con rata ni ratón me temo ...

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    Thank you for your inquiry.

    For ab8297, we have never tested this antibody for cross-reactivity with mouse or rat. Therefore, we would not be able to guarantee it for non-cross-reactivity with mouse and rat.

    For ab108326, this antib...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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