Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free (ab46707)


  • Product nameAnti-Insulin antibody [IN-05] - Low endotoxin, Azide free
    See all Insulin primary antibodies
  • Description
    Mouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free
  • SpecificityThe antibody blocks binding of insulin to the receptor.
  • Tested applicationsSuitable for: ELISA, RIA, ICC/IF, IHC-P, Functional Studiesmore details
  • Species reactivity
    Reacts with: Cow, Human, Pig
  • Immunogen

    Porcine insulin

  • Positive control
    • IHC-P: Human pancreas tissue sections.
  • General notes

    Preserved by filter sterilization



Our Abpromise guarantee covers the use of ab46707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Blocks the binding of insulin to the receptor.


  • FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the insulin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free images

  • IHC image of Insulin staining in human pancreas formalin fixed paraffin embedded tissue section (ab4611), performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46707, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab46707 staining Insulin in human beta cells grown in vitro in extracellular matrix by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using Triton X-100, then blocked with 2% serum for 2 hours at 25°C. Samples were then incubated with ab46707 at a 1/100 dilution for 20 minutes at 4°C. The secondary used was a Cy3 conjugated goat anti-mouse IgG (ab97035), used at a 1/100 dilution. The nuclei were stained with DAPI.

    See Abreview

References for Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free (ab46707)

ab46707 has not yet been referenced specifically in any publications.

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While we have a number of anti-Insulin antibodies which you would be able to conjugated to an organic fluorophore, we currently do not offer AlexaFluor 500 in our EasyLink conjugation kits. We do offer a number of...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human beta cells)
Specification Human beta cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

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Submitted Jun 20 2011