Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free (ab46707)
Key features and details
- Mouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free
- Suitable for: ELISA, IHC-P, ICC/IF, Functional Studies, RIA
- Reacts with: Cow, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free
See all Insulin primary antibodies -
Description
Mouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free -
Host species
Mouse -
Specificity
The antibody blocks binding of insulin to the receptor.
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Tested applications
Suitable for: ELISA, IHC-P, ICC/IF, Functional Studies, RIAmore details -
Species reactivity
Reacts with: Cow, Human, Pig -
Immunogen
Full length protein corresponding to Pig Insulin. Full Length Native Protein Porcine insulin
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General notes
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. Preserved by filter sterilization. -
Clonality
Monoclonal -
Clone number
IN-05 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
Blocks the binding of insulin to the receptor. |
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RIA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. Blocks the binding of insulin to the receptor. |
RIA
Use at an assay dependent concentration. |
Target
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Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. -
Involvement in disease
Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3630 Human
- Entrez Gene: 397415 Pig
- Omim: 176730 Human
- SwissProt: P01317 Cow
- SwissProt: P01308 Human
- SwissProt: P01315 Pig
- Unigene: 272259 Human
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Alternative names
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
see all
Datasheets and documents
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Datasheet download
References (5)
ab46707 has been referenced in 5 publications.
- Ren R et al. Nanopore extended field-effect transistor for selective single-molecule biosensing. Nat Commun 8:586 (2017). PubMed: 28928405
- Woods CA et al. Insulin receptor activation in the nucleus accumbens reflects nutritive value of a recently ingested meal. Physiol Behav 159:52-63 (2016). PubMed: 26988281
- England E et al. Application of the mirrorball high-sensitivity cytometer to multiplexed assays for antibody drug discovery. J Biomol Screen 20:536-44 (2015). PubMed: 25381256
- Stouffer MA et al. Insulin enhances striatal dopamine release by activating cholinergic interneurons and thereby signals reward. Nat Commun 6:8543 (2015). PubMed: 26503322
- Okere B et al. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmacol 28:390-402 (2015). PubMed: 26216908