Overview

  • Product nameAnti-Insulin degrading enzyme / IDE antibody
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description
    Rabbit polyclonal to Insulin degrading enzyme / IDE
  • Specificityab28561 recognises the inactive metalloproteinase domain of IDE.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide based on the inactive metalloproteinase domain of human Insulin degrading enzyme/ IDE. (Peptide available as ab41259.)

Properties

Applications

Our Abpromise guarantee covers the use of ab28561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 118 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.

Target

  • FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • Sequence similaritiesBelongs to the peptidase M16 family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody images

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab28561) at 1/1000 dilution

    Lane 1 : Insulysin at 0.05 µg
    Lane 2 : Insulysin at 0.01 µg
    Lane 3 : Insulysin at 0.001 µg


    Predicted band size : 118 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

References for Anti-Insulin degrading enzyme / IDE antibody (ab28561)

This product has been referenced in:
  • Miners JS  et al. Immunocapture-based fluorometric assay for the measurement of insulin-degrading enzyme activity in brain tissue homogenates. J Neurosci Methods 169:177-81 (2008). ELISA ; Human . Read more (PubMed: 18221786) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"