Anti-Insulin degrading enzyme / IDE antibody (ab32216)

Western blot - Insulin degrading enzyme / IDE antibody (ab32216)

All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml

Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg

Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 118 kDa
Observed band size : 118 kDa

Immunohistochemistry - Free Floating - Insulin degrading enzyme / IDE antibody (ab32216)

Immunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

Sophie Pezet,CNRS, Paris

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Insulin degrading enzyme / IDE antibody (ab32216)

Image courtesy of Human Protein Atlas

ab32216 staining human Gall bladder. The paraffin embedded human skin tissue was incubated with ab32216 (1/50 dilution) for 30 mins at room temperature. The staining is predominantly cytoplasmic although some nuclear staining can be seen. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab32216 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

Further results for this antibody can be found at www.proteinatlas.org

Immunocytochemistry/ Immunofluorescence - Anti-Insulin degrading enzyme / IDE antibody (ab32216)

ab32216 staining cultured rat OCP by ICC/IF. The cultured cells were fixed with paraformaldehyde and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured cellswere then stained with ab322161 at 1/500 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue.

This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhury