Recombinant HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099]
See all Insulin degrading enzyme / IDE primary antibodies -
Description
HRP Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2, A375, and K562 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6099 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa). |
Target
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Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
Sequence similarities
Belongs to the peptidase M16 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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Database links
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q9JHR7 Mouse
- SwissProt: P35559 Rat
- Unigene: 500546 Human
see all -
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Images
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All lanes : HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IDE (Insulin degrading enzyme / IDE) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 20 minutesab201836 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells as signal was lost in IDE (Insulin degrading enzyme / IDE) knockout cells. Wild-type and IDE (Insulin degrading enzyme / IDE) knockout samples were subjected to SDS-PAGE. Ab201836 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab201836) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : A375 (Human melanoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201836 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab201836 has not yet been referenced specifically in any publications.