Anti-Insulin degrading enzyme / IDE antibody (ab63137)


  • Product nameAnti-Insulin degrading enzyme / IDE antibody
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description
    Rabbit polyclonal to Insulin degrading enzyme / IDE
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the Center region of human Insulin degrading enzyme/ IDE.

  • Positive control
    • A375 cell line lysates and human hepatocarcinoma tissue.



Our Abpromise guarantee covers the use of ab63137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
ELISA 1/1000.
IHC-P 1/50.


  • FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • Sequence similaritiesBelongs to the peptidase M16 family.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Anti-Insulin degrading enzyme / IDE antibody images

  • Anti-Insulin degrading enzyme / IDE antibody (ab63137) at 1/60 dilution + A375 cell line lysates at 35 µg

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of Insulin degrading enzyme / IDE expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/50 ab63137, followed by peroxidase-conjugated secondary antibody step and DAB staining.
  • ICC/IF image of ab63137 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Insulin degrading enzyme / IDE antibody (ab63137)

ab63137 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab63137.
Please use the links above to contact us or submit feedback about this product.