The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/50 - 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similaritiesBelongs to the peptidase M16 family.
Post-translational modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
Immunohistochemical analysis of Insulin degrading enzyme / IDE expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/50 ab63137, followed by peroxidase-conjugated secondary antibody step and DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Insulin degrading enzyme / IDE antibody (ab63137)
ICC/IF image of ab63137 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63137, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Insulin degrading enzyme / IDE antibody (ab63137)
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