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Anti-Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560)

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Overview

Product name

Anti-Insulin degrading enzyme / IDE antibody - Catalytic domain
See all Insulin degrading enzyme / IDE products (11) ...

Description

Rabbit polyclonal to Insulin degrading enzyme / IDE - Catalytic domain

Specificity

ab28560 recognises the Catalytic Metalloproteinase domain of IDE

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide based on the catalytic metalloproteinase domain of human Insulin degrading enzyme / IDE. (Peptide available as ab41257.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> Insulysin
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

  • Western blot - Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560)Western blot - Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 118 kDa.(A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.)

Target

Function

Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

Sequence similarities

Belongs to the peptidase M16 family.

Post-translational
modifications

The N-terminus is blocked.

Cellular localization

Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

Target information above from: UniProt accessionP14735 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Abeta-degrading protease antibody
  • FLJ35968 antibody
  • Ide antibody
  • IDE_HUMAN antibody
  • insulin protease antibody
  • Insulin-degrading enzyme antibody
  • insulinase antibody
  • insulysin antibody
  • OTTHUMP00000020097 antibody
see all

Database links

see all

Anti-Insulin degrading enzyme / IDE antibody - Catalytic domain images:

  Western blot - Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560)

Western blot - Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560)

All lanes : Anti-Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560) at 1/1000 dilution

Lane 1 : Insulysin at 0.05 µg
Lane 2 : Insulysin at 0.01 µg
Lane 3 : Insulysin at 0.001 µg


Predicted band size : 118 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa (possible cleavage fragment).

Protocols

  • Recommended WB protocol

    • References for Anti-Insulin degrading enzyme / IDE antibody - Catalytic domain (ab28560)

    ab28560 has not yet been referenced specifically in any publications.

    Publishing research using ab28560? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"