Validated using a knockout cell line
Recombinant
RabMAb

Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538)

Overview

  • Product name
    Anti-Insulin degrading enzyme / IDE antibody [EPR6099]
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description
    Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • HeLa, HepG2, A375, and K562 cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 118 kDa.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
    • Sequence similarities
      Belongs to the peptidase M16 family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abeta-degrading protease antibody
      • FLJ35968 antibody
      • Ide antibody
      • IDE_HUMAN antibody
      • Insulin protease antibody
      • Insulin-degrading enzyme antibody
      • Insulinase antibody
      • Insulysin antibody
      • OTTHUMP00000020097 antibody
      see all

    Images

    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Insulin degrading enzyme / IDE with purified ab109538 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.



    • Predicted band size : 118 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IDE knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109538 observed at 118 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109538 was shown to specifically react with IDE when IDE knockout samples were used. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab109538 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (ab109538) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 118 kDa

    References

    ab109538 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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