Anti-Insulin + Proinsulin antibody [D3E7] (ab8305)

Overview

  • Product nameAnti-Insulin + Proinsulin antibody [D3E7]
    See all Insulin + Proinsulin primary antibodies
  • Description
    Mouse monoclonal [D3E7] to Insulin + Proinsulin
  • SpecificityKd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin
  • Tested applicationsSuitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • General notes


    Detection of insulin and proinsulin

Properties

Applications

Our Abpromise guarantee covers the use of ab8305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

References for Anti-Insulin + Proinsulin antibody [D3E7] (ab8305)

ab8305 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your e-mail. I would like to confirm that the immunogen was purified human insulin.

Thank you for your enquiry. This antibody was tested along with ab8304 and ab28063 in sandwich ELISAs against recombinant proinsulin and insulin. ab8305 and ab8304 are directed to different antigenic determinants. The Kd for ab8304 is 8,1 x 10-8...

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Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

Thank you for your enquiry. This product has not been tested for use against recombinant human insulin. Ab8305 has only been tested with purified human insulin and proinsulin. If you have any further questions then please get back in touch with us.

Thank you for your enquiry. The antibody reacts with whole molecule of mouse insulin and proinsulin. The antibody is working in immunohistochemistry on frozen sections. As immunogen we have used native human insulin.

Thank you for your enquiry. Our anti-rat/mouse insulin/proinsulin recognizes rat/mouse insulin with the same efficacy as human insulin or proinsulin.

The immunogen used was purified human insulin.

The customer did not use this antibody in FACS for their review.

Thank you for your enquiry. Ab8305 has been tested for application in ELISA and shown to cross-react with Rat. Ab6728 is a compatible secondary for ab8305. So yes, they should be suitable for your interests. If you have any more questions, please conta...

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Thank you for your enquiry. All the information we have regarding ab8305 is located on the online datasheet. We do not have an ideal dilution for IHC. I suggest that you consult the IHC general protocol section which may be helpful. If you do end up us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"