Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756)

Overview

  • Product nameAnti-Insulin + Proinsulin antibody [D3E7] (Biotin)
    See all Insulin + Proinsulin primary antibodies
  • Description
    Mouse monoclonal [D3E7] to Insulin + Proinsulin (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Full length protein (Human).

  • General notesFor concentration (OD280nm, E0.1% = 1.4). Affinity constant Kd = 6.3 x 10E-8M.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab20756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Can be paired for sandwich ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).

ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).

Target

Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) images

  • ab20756 staining Insulin + Proinsulin in human pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C followed by incubation with Cy3®-conjugated streptavidin for 30 minutes in the dark at room temperature. Nuclei were counterstained with DAPI.

    See Abreview

References for Anti-Insulin + Proinsulin antibody [D3E7] (Biotin) (ab20756)

This product has been referenced in:
  • Kuhn C  et al. Human CD3 transgenic mice: preclinical testing of antibodies promoting immune tolerance. Sci Transl Med 3:68ra10 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21289272) »
  • Yang RY  et al. Ablation of a galectin preferentially expressed in adipocytes increases lipolysis, reduces adiposity, and improves insulin sensitivity in mice. Proc Natl Acad Sci U S A 108:18696-701 (2011). ELISA ; Mouse . Read more (PubMed: 21969596) »

See all 2 Publications for this product

Product Wall

This antibody is already conjugated to biotin and therefore does not need a secondary antibody for detection.
What you will need is avidin or streptavidin conjugated to an enzyme or fluorochrome that will bind to multiple sites on the biotinylated...

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Les anticorps utilisés dans le kit ELISA ab100578 sont les anticorps ab8304 comme anticorps de capture et ab20756 comme anticorps de détection. Ces deux anticorps sont testés et garantis chez le rat. Le kit lui-même...

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Thank you for your inquiry. 
The antibody pair that we use in the Human Insulin ELISA Kit (ab100578) is capture antibody (ab8304) and detection antibody (ab20756). 
http://www.abcam.com/insulin--proinsulin-antibody-d6c4-ab8304.html
http:/...

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Thank you for your further enquiry.

I am sorry to confirm that as far as we are aware, ab8304 and ab20756 insulin antibodies have never been tested in ELISPOT. All tested and guaranteed applications are specified on our datasheets, and these...

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Thank you for your enquiry which has been forwarded to the scientific support team. We can be contacted at mailto:technical@abcam.com, or directly from the website.

I can confirm that ab8304 can be paired for Sandwich ELISA with Mouse monocl...

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Thank you for contacting us. Yes, the clones D6C4 and D3E7 have been validated for use as a sandwich ELISA pair and will be guaranteed for that purpose. Because the detection antibody you are choosing is already biotinylated, it will not be an issue th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Titox x100
Blocking step Rouche Blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Nov 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Pancreas)
Permeabilization Yes - 0.25% Triton x100
Specification Pancreas
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3%
Username

Dr. Anahi Chavarria

Verified customer

Submitted Sep 15 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"