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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab36550 as a replacement.
Our Abpromise guarantee covers the use of ab982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde or methanol fixed)
|Blocking||Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system. Inhibition of insulin binding: IM-9 lymphocytes = 92%, Adipocytes = 98% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM|
|WB||Use at an assay dependent concentration. PubMed: 23048032|