Anti-Insulin Receptor alpha antibody [47-9] (ab982)


  • Product nameAnti-Insulin Receptor alpha antibody [47-9]
    See all Insulin Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [47-9] to Insulin Receptor alpha
  • SpecificityThis antibody reacts specifically with the alpha subunit of the insulin receptor.
  • Tested applicationsSuitable for: Flow Cyt, Blocking, WBmore details
  • Species reactivity
    Reacts with: Rabbit, Cow, Human
  • Immunogen

    IM-9 lymphocytes.



Our Abpromise guarantee covers the use of ab982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. (paraformaldehyde or methanol fixed)

Blocking Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system. Inhibition of insulin binding: IM-9 lymphocytes = 92%, Adipocytes = 98% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM
WB Use at an assay dependent concentration. PubMed: 23048032


  • RelevanceThe human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
  • Cellular localizationMembrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • CD220 antibody
    • HHF5 antibody
    • HIR A antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit alpha antibody
    • IR antibody
    see all

Anti-Insulin Receptor alpha antibody [47-9] images

  • Overlay histogram showing Jurkat cells stained with ab982 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab982, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Flow cytometry analysis of Human myeloma cells, staining Insulin Receptor alpha with ab982. A phycoethrin-conjugated anti-mouse IgG was used as the secondary antibody.

References for Anti-Insulin Receptor alpha antibody [47-9] (ab982)

This product has been referenced in:
  • Murakami J  et al. Involvement of insulin-like peptide in long-term synaptic plasticity and long-term memory of the pond snail Lymnaea stagnalis. J Neurosci 33:371-83 (2013). Read more (PubMed: 23283349) »
  • Purushothaman A  et al. Heparanase Enhances the Insulin Receptor Signaling Pathway to Activate Extracellular Signal-regulated Kinase in Multiple Myeloma. J Biol Chem 287:41288-96 (2012). WB, Flow Cyt ; Human . Read more (PubMed: 23048032) »

See all 6 Publications for this product

Product Wall

We have not done blocking tests ourselves but the two earliest papers listed on the datasheet have discussions of binding/blocking assays. Soos et al 1986 has some data in figure 2 that suggests the amount of inhibition will be sample-dependent, and th...

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The concentration of ˜3.5 µM stated on the datasheet of ab982 refers to an antibody concentration of 0.52 mg/ml. The current lots we have in stock are 0.92 and 1.2 mg/ml. The concentration of these can be calculated by taking the molecular w...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Hamster Cell lysate - whole cell (Ovary)
Total protein in input 50 µg
Specification Ovary
Immuno-precipitation step Protein A/G

Abcam user community

Verified customer

Submitted Nov 21 2007

Thank you for getting back in touch with me. I can confirm that the concentration of this antibody is 3.5 uM. Apolagies for not updating the datasheet correctly. Good luck with your research!

Thank you for getting back to me. I apolagise for the confusion. I was provided with the incorrect information. The concentration of the antibody should read 3.5uM. I have updated the datasheet to this effect. I hope this information helps, plea...

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Thank you for your enquiry. Further to correspondence with the source of this antibody I can confirm that this antibody does NOT cross-react with IGF receptors. Furthermore I can confirm that the concentration of the antibody is approximately 0.3...

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Thank you for your enquiry and patience. The concentration for half-maximum inhibition of 125I-insulin binding to adipocytes by ab982 was 200 pM. Full details of this experiment and the resulting data can be found in the following reference (the free f...

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