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Our Abpromise guarantee covers the use of ab982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde or methanol fixed)
|Blocking||Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system. Inhibition of insulin binding: IM-9 lymphocytes = 92%, Adipocytes = 98% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM|
|WB||Use at an assay dependent concentration. PubMed: 23048032|