Overview

  • Product nameAnti-Insulin Receptor alpha antibody [83-14]
    See all Insulin Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [83-14] to Insulin Receptor alpha
  • Tested applicationsSuitable for: Functional Studies, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Sheep, Cow, Human, Pig
    Does not react with: Mouse, Rat, Rabbit
  • Immunogen

    IM-9 lymphocytes followed by purified insulin receptor.

  • EpitopeAmino acids 469 - 592 (exon 7 and 8).
  • Positive control
    • IM-9 lymphocyte cells or Placenta .

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. Can be used to inhibit insulin binding and for tyrosine kinase assays.
IP Use at 2 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 18411068

Target

  • RelevanceThe human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
  • Cellular localizationMembrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • CD220 antibody
    • HHF5 antibody
    • HIR A antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit alpha antibody
    • IR antibody
    see all

Anti-Insulin Receptor alpha antibody [83-14] images

  • ab981, staining Insulin Receptor alpha in Human skin fibroblast cells, by Immunocytochemistry/ Immunofluorescence.

    Fibroblasts cultured from skin biopsies of a patient with Rabson–Mendenhall syndrome and a healthy control, were fixed in methanol. Samples were fixed with primary antibody and a FITC-labeled donkey polyclonal mouse antibody (ab7057) was used as a secondary antibody. DNA was counterstained with DAPI.

References for Anti-Insulin Receptor alpha antibody [83-14] (ab981)

This product has been referenced in:
  • Thiel CT  et al. Two novel mutations in the insulin binding subunit of the insulin receptor gene without insulin binding impairment in a patient with Rabson-Mendenhall syndrome. Mol Genet Metab 94:356-62 (2008). ICC/IF ; Human . Read more (PubMed: 18411068) »
  • Zhang B & Roth RA A region of the insulin receptor important for ligand binding (residues 450-601) is recognized by patients' autoimmune antibodies and inhibitory monoclonal antibodies. Proc Natl Acad Sci U S A 88:9858-62 (1991). Read more (PubMed: 1719540) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"