The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Can be used to inhibit insulin binding and for tyrosine kinase assays.
Use at 2 µg/mg of lysate.
Use at an assay dependent concentration. PubMed: 18411068
RelevanceThe human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism.
Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
Cellular localizationMembrane; single pass type I membrane protein.
Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor alpha antibody [83-14] (ab981)Image from Thiel CT et al., Mol Genet Metab. 2008 Jul;94(3):356-62. Epub 2008 Apr 14. Fig 4.; doi:10.1016/j.ymgme.2008.02.013; 14 April 2008, Molecular Genetics and Metabolism, 94 (3), 356–362, with permission from Elsevier.
ab981, staining Insulin Receptor alpha in Human skin fibroblast cells, by Immunocytochemistry/ Immunofluorescence.
Fibroblasts cultured from skin biopsies of a patient with Rabson–Mendenhall syndrome and a healthy control, were fixed in methanol. Samples were fixed with primary antibody and a FITC-labeled donkey polyclonal mouse antibody (ab7057) was used as a secondary antibody. DNA was counterstained with DAPI.
References for Anti-Insulin Receptor alpha antibody [83-14] (ab981)
This product has been referenced in:
Thiel CT et al. Two novel mutations in the insulin binding subunit of the insulin receptor gene without insulin binding impairment in a patient with Rabson-Mendenhall syndrome. Mol Genet Metab94:356-62 (2008).
Read more (PubMed: 18411068) »
Zhang B & Roth RA A region of the insulin receptor important for ligand binding (residues 450-601) is recognized by patients' autoimmune antibodies and inhibitory monoclonal antibodies. Proc Natl Acad Sci U S A88:9858-62 (1991).
Read more (PubMed: 1719540) »