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Transfected 3T3 cells overexpressing the human Insulin Receptor alpha
The antibody enhances the binding of 125I insulin binding to the insulin receptor of HIR3.5/3T3 cells and stimulates insulin mediated 3H thymidine incorporation in these cells.
Our Abpromise guarantee covers the use of ab36550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde or methanol fixed cells)
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|