Anti-Insulin Receptor alpha antibody [83-7] (Biotin) (ab79211)

Overview

  • Product nameAnti-Insulin Receptor alpha antibody [83-7] (Biotin)
    See all Insulin Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [83-7] to Insulin Receptor alpha (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Functional Studies, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Human, Pig
    Does not react with: Mouse, Rat
  • Immunogen

    IM9 lymphocytes followed by IR.

  • EpitopeExon 3
  • Positive control
    • IM9 lymphocyte cells. Placenta tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
IHC-P
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    Tyrosine Kinase Assay: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 minutes at room temperature.
    Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris HCl, pH 2.0 for 15 minutes at room temperature or 10 minutes at 37 °C

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins.
    • Cellular localizationMembrane; single pass type I membrane protein.
    • Database links
    • Alternative names
      • CD220 antibody
      • HHF5 antibody
      • HIR A antibody
      • INSR antibody
      • Insulin receptor antibody
      • Insulin receptor subunit alpha antibody
      • IR antibody
      see all

    References for Anti-Insulin Receptor alpha antibody [83-7] (Biotin) (ab79211)

    This product has been referenced in:
    • Macaulay SL  et al. Mutagenic structure/function analysis of the cytoplasmic cysteines of the insulin receptor. Biochem J 306 ( Pt 3):811-20 (1995). Flow Cyt . Read more (PubMed: 7702578) »
    • Soos MA  et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J 235:199-208 (1986). Human, Cow, Pig, Rabbit, Sheep . Read more (PubMed: 2427071) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting Abcam about ab79211.
    Please find the answers to your questions below:
    The product’s clone origin is Swiss Prot #P06213 http://www.uniprot.org/uniprot/P06213
    Questions:
    What part of the protein would th...

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    Thank you for your inquiry.

    The epitope for this product is Exon 3. As I am not familar to what region this exon translates to, I have contacted the lab for more assistance with your inquiry. I will let you know as soon as they get back to m...

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    Thank you for contacting us.

    The originator of this product has reported that this antibody does not significantly inhibit Insulin binding as per the publication bySoos MA, et. al. Biochem J, 1986, 235(1):199-208.

    I hope this ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"