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Human placental insulin receptor.
Our Abpromise guarantee covers the use of ab983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18411068|
|Blocking||Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system.|
|Functional Studies||Use at an assay dependent concentration. Inhibition of insulin binding: IM-9 lymphocytes = 35%, Adipocytes = 90% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM, Stimulation of lipogenesis = 0.3nM. This antibody has insulin-like biological activity and therefore activates the receptor kinase.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|