Anti-Insulin Receptor beta antibody [18-44] (ab983)


  • Product nameAnti-Insulin Receptor beta antibody [18-44]
    See all Insulin Receptor beta primary antibodies
  • Description
    Mouse monoclonal [18-44] to Insulin Receptor beta
  • SpecificityThis antibody reacts specifically with the beta subunit of the insulin receptor.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, Blocking, Functional Studies, IP, WBmore details
  • Species reactivity
    Reacts with: Rabbit, Cow, Human
  • Immunogen

    Human placental insulin receptor.



Our Abpromise guarantee covers the use of ab983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration. PubMed: 18411068
Blocking Use at an assay dependent concentration. Dilute in PBS or medium which is identical to that used in the assay system.
Functional Studies Use at an assay dependent concentration. Inhibition of insulin binding: IM-9 lymphocytes = 35%, Adipocytes = 90% Concentration for half maximal effect: Inhibition of insulin binding = 0.2nM, Stimulation of lipogenesis = 0.3nM. This antibody has insulin-like biological activity and therefore activates the receptor kinase.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.


  • RelevanceInsulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
  • Cellular localizationMembrane; Single pass type I membrane protein.
  • Database links
  • Alternative names
    • CD220 antibody
    • HHF5 antibody
    • HIR B antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit beta antibody
    • IR antibody
    see all

Anti-Insulin Receptor beta antibody [18-44] images

  • Overlay histogram showing HepG2 cells stained with ab983 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab983, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab983, staining Insulin Receptor beta in Human skin fibroblast cells, by Immunocytochemistry/ Immunofluorescence.

    Fibroblasts cultured from skin biopsies of a patient with Rabson–Mendenhall syndrome and a healthy control, were fixed in methanol. Samples were fixed with primary antibody and a FITC-labeled donkey polyclonal mouse antibody (ab7057) was used as a secondary antibody. DNA was counterstained with DAPI.

References for Anti-Insulin Receptor beta antibody [18-44] (ab983)

This product has been referenced in:
  • Yosten GL  et al. Evidence for an interaction between proinsulin C-peptide and GPR146. J Endocrinol 218:B1-8 (2013). ICC/IF . Read more (PubMed: 23759446) »
  • Thiel CT  et al. Two novel mutations in the insulin binding subunit of the insulin receptor gene without insulin binding impairment in a patient with Rabson-Mendenhall syndrome. Mol Genet Metab 94:356-62 (2008). ICC/IF ; Human . Read more (PubMed: 18411068) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Mouse Tissue lysate - other (Hep G2)
Specification Hep G2
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 27 2014

According to the Uniprot homepage the alpha chain is located from amino acid 28-758, whereas the beta chain is from aa 763-1382:

ab69508 has its immunogen in the cytoplasmic domain of ...

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Thank you very much for having provided your feedback in the last survey.

I am happy to hear that you were satisfied with our service and that the ab83 does work better than the original antibody received. I am sorry though, that the ab83 sti...

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Thank you for your inquiry and your patience with this reply. We have limited data regarding the specific epitope for this antibody, but it appears that it is amino acids 765-770 (PEEHRP). I hope this information helps. Please contact us with an...

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Thanks for your email and I'm sorry to hear that your customer is experiencing difficulty with this antibody. I do require some additional details in order to investigate this. Exactly what type of samples were used? And from which species? How was...

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Thank you for your enquiry. The reactivity of this antibody with bovine and rabbit insulin receptor is extremely weak. In Western blots, a very faint band can be seen in some samples, but this banding is not seen consistently in all samples. We menti...

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Thank you for your enquiry. This antibody produces very faint banding when used at relatively high concentration (25 ug/ml) to immunoblot bovine or rabbit insulin receptor. In contrast, immunoblotting on human insulin receptor produces a sharp, strong...

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