Anti-Insulin Receptor beta antibody [CT-1] (ab80527)


  • Product name
    Anti-Insulin Receptor beta antibody [CT-1]
    See all Insulin Receptor beta primary antibodies
  • Description
    Mouse monoclonal [CT-1] to Insulin Receptor beta
  • Specificity
    Reacts with autophosphorylated and unoccupied receptors as well as the receptor insulin complex. Does not affect insulin stimulated autophosphorylation.
  • Tested applications
    Suitable for: ELISA, IP, Purification, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 1356-1370 of Human Insulin Receptor beta

  • Epitope
    aa -GRVLTLPRS- within the C terminal.
  • Positive control
    • IM-9 lymphocyte cells.



Our Abpromise guarantee covers the use of ab80527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at 2 µg/mg of lysate.
Purification Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution. Tyrosine kinase assay


  • Relevance
    Insulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
  • Cellular localization
    Membrane; Single pass type I membrane protein.
  • Database links
  • Alternative names
    • CD220 antibody
    • HHF5 antibody
    • HIR B antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit beta antibody
    • IR antibody
    see all


This product has been referenced in:
  • Ganderton RH  et al. A monoclonal anti-peptide antibody reacting with the insulin receptor beta-subunit. Characterization of the antibody and its epitope and use in immunoaffinity purification of intact receptors. Biochem J 288 ( Pt 1):195-205 (1992). AP, IP ; Human, Mouse, Rat . Read more (PubMed: 1280110) »
  • Prigent SA  et al. Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies. J Biol Chem 265:9970-7 (1990). AP ; Human . Read more (PubMed: 1693619) »

See all 2 Publications for this product

Customer reviews and Q&As

According to the Uniprot homepage the alpha chain is located from amino acid 28-758, whereas the beta chain is from aa 763-1382:

ab69508 has its immunogen in the cytoplasmic domain of ...

Read More


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