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Synthetic peptide (Human). Synthetic phosphopeptide derived from the region of the human Insulin Receptor that contains tyrosine 972 (as numbered according to Ebina, et al. (tyrosine 960 according to Ullrich, et al.).
Biological actions of insulin are mediated by the Insulin Receptor (IR), a receptor tyrosine kinase that regulates multiple signaling pathways through activation of a series of phosphorylation cascades. The IR is a heterotetrameric protein consisting of two ligand-binding alpha subunits and two beta subunits that each contain a tyrosine kinase domain. Insulin binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. Tyrosine 972 is in the juxtamembrane Asn-Pro- Glu-Tyr (NPEY) motif. Phosphorylation of IR tyrosine 972 is required for the binding and/or phosphorylation of the adapter protein Shc, the PTB domain, IRS-1, PI3 kinase, and the Suppressor of Cytokine Signaling (SOCS).
Our Abpromise guarantee covers the use of ab5678 in the following tested applications.
|IP||Use at an assay dependent dilution. PubMed: 17264162|
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 110 kDa.|