Overview

  • Product nameAnti-Insulin Receptor R antibody [EP327Y]
    See all Insulin Receptor R primary antibodies
  • Description
    Rabbit monoclonal [EP327Y] to Insulin Receptor R
  • Specificityab40782 recognises insulin receptor related receptor (IRR).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human IRR alpha subunit.

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab40782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa.
IHC-P 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionThis receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 3 fibronectin type-III domains.
      Contains 1 protein kinase domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • INSRR antibody
      • INSRR_HUMAN antibody
      • Insulin receptor related receptor precursor antibody
      • Insulin receptor-related protein alpha chain antibody
      • Insulin receptor-related protein beta chain antibody
      • insulin receptor-related receptor antibody
      • IR R antibody
      • IR related receptor antibody
      • IR-related receptor antibody
      • IRR antibody
      • Sir r antibody
      • Sirr antibody
      see all

    Anti-Insulin Receptor R antibody [EP327Y] images

    • All lanes : Anti-Insulin Receptor R antibody [EP327Y] (ab40782) at 1/2000 dilution

      Lane 1 : PC12
      Lane 2 : MCF7
      Lane 3 : Hela
      Lane 4 : SHSY5Y

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 80, 144 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,37 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.

    References for Anti-Insulin Receptor R antibody [EP327Y] (ab40782)

    This product has been referenced in:
    • Li Y  et al. LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and kidney malformations in Cenani-Lenz syndrome. Am J Hum Genet 86:696-706 (2010). WB ; Human . Read more (PubMed: 20381006) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"