Overview

  • Product name
    INT dye
  • Description
    Tetrazolium dye precursor used to measure dehydrogenase activity.
  • Alternative names
    • 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride
    • INT
    • Iodonitrotetrazolium chloride
    • Iodonitrotetrazolium purple
    • Iodonitrotetrazolum violet
    • p-Iodonitrotetrazolium Violet
    see all
  • Biological description

    Tetrazolium dye precursor that forms a purple formazan dye on reduction. Used as an electron acceptor for the colorimetric assay of dehydrogenase activity. Also used for western blot staining and as a staining agent for soft agar colony formation assays.

  • Purity
    > 98%

Properties

  • Chemical name
    2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride
  • Molecular weight
    505.71
  • Chemical structure
    Chemical Structure
  • Molecular formula
    C19H13ClIN5O2
  • CAS Number
    146-68-9
  • Storage instructions
    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:
  • Berridge MV  et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 11:127-52 (2005). Read more (PubMed: 16216776) »
  • Green JD & Narahara HT Assay of succinate dehydrogenase activity by the tetrazolium method: evaluation of an improved technique in skeletal muscle fractions. J Histochem Cytochem 28:408-12 (1980). Read more (PubMed: 6966645) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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