Tetrazolium dye precursor that forms a purple formazan dye on reduction. Used as an electron acceptor for the colorimetric assay of dehydrogenase activity. Also used for western blot staining and as a staining agent for soft agar colony formation assays.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Berridge MV et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev11:127-52 (2005).
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Green JD & Narahara HT Assay of succinate dehydrogenase activity by the tetrazolium method: evaluation of an improved technique in skeletal muscle fractions. J Histochem Cytochem28:408-12 (1980).
Read more (PubMed: 6966645) »