Overview

  • Product name
  • Description
    Rabbit polyclonal to INT11
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding a region between residues 550 and 600 of human INT11 (NP_060341.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab75276 was affinity purified using an epitope specific to INT11 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.
  • Sequence similarities
    Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
  • Domain
    The HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410006F12Rik antibody
    • AU044035 antibody
    • Cleavage and polyadenylation specific factor 3 like antibody
    • Cleavage and polyadenylation-specific factor 3-like protein antibody
    • CPSF3 like protein antibody
    • CPSF3-like protein antibody
    • CPSF3L antibody
    • CPSF73L antibody
    • FLJ13294 antibody
    • FLJ20542 antibody
    • Human integrator complex subunit 11 antibody
    • INT 11 antibody
    • Int11 antibody
    • INT11_HUMAN antibody
    • Integrator complex subunit 11 antibody
    • INTS 11 antibody
    • INTS11 antibody
    • MGC124975 antibody
    • OTTHUMP00000044092 antibody
    • Protein related to CPSF subunits of 68 kDa antibody
    • RC 68 antibody
    • RC 68, related antibody
    • RC-68 antibody
    • RC68 antibody
    • Related to CPSF subunits 68 kDa antibody
    • Related to CPSF subunits, 68 kDa protein antibody
    • RGD1306841 antibody
    • RP23 242K15.19 antibody
    see all

Anti-INT11 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INT11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-INT11 antibody (ab75276) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 68 kDa


    Exposure time : 3 minutes
  • Detection of INT11 by Western Blot of Immunprecipitate.
    ab75278 at 1µg/ml staining INT11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-INT11 antibody (ab75276)

This product has been referenced in:
  • Xie M  et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev 29:1552-64 (2015). Read more (PubMed: 26220997) »
  • O'Reilly D  et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24097444) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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