Overview

  • Product nameAnti-INT11 antibody
    See all INT11 primary antibodies
  • Description
    Rabbit polyclonal to INT11
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding a region between residues 550 and 600 of human INT11 (NP_060341.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab75276 was affinity purified using an epitope specific to INT11 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.
  • Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
  • DomainThe HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410006F12Rik antibody
    • AU044035 antibody
    • Cleavage and polyadenylation specific factor 3 like antibody
    • Cleavage and polyadenylation-specific factor 3-like protein antibody
    • CPSF3 like protein antibody
    • CPSF3-like protein antibody
    • CPSF3L antibody
    • CPSF73L antibody
    • FLJ13294 antibody
    • FLJ20542 antibody
    • Human integrator complex subunit 11 antibody
    • INT 11 antibody
    • Int11 antibody
    • INT11_HUMAN antibody
    • Integrator complex subunit 11 antibody
    • INTS 11 antibody
    • INTS11 antibody
    • MGC124975 antibody
    • OTTHUMP00000044092 antibody
    • Protein related to CPSF subunits of 68 kDa antibody
    • RC 68 antibody
    • RC 68, related antibody
    • RC-68 antibody
    • RC68 antibody
    • Related to CPSF subunits 68 kDa antibody
    • Related to CPSF subunits, 68 kDa protein antibody
    • RGD1306841 antibody
    • RP23 242K15.19 antibody
    see all

Anti-INT11 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INT11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-INT11 antibody (ab75276) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 68 kDa


    Exposure time : 3 minutes
  • Detection of INT11 by Western Blot of Immunprecipitate.
    ab75278 at 1µg/ml staining INT11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-INT11 antibody (ab75276)

This product has been referenced in:
  • O'Reilly D  et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24097444) »
  • Xie M  et al. Mammalian 5'-capped microRNA precursors that generate a single microRNA. Cell 155:1568-80 (2013). Read more (PubMed: 24360278) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab75276.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"