The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCatalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage.
Sequence similaritiesBelongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.
DomainThe HXHXDH motif is essential for the endoribonuclease activity of the CPSF complex.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INT11 with ab75276 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - INT11 antibody (ab75276)
All lanes : Anti-INT11 antibody (ab75276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 68 kDa Observed band size : 68 kDa
Exposure time : 3 minutes
Immunoprecipitation - INT11 antibody (ab75276)
Detection of INT11 by Western Blot of Immunprecipitate. ab75278 at 1µg/ml staining INT11 in HeLa whole cell lysate immunoprecipitated using ab75276 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-INT11 antibody (ab75276)
This product has been referenced in:
Xie M et al. The host Integrator complex acts in transcription-independent maturation of herpesvirus microRNA 3' ends. Genes Dev29:1552-64 (2015).
Read more (PubMed: 26220997) »
O'Reilly D et al. Human snRNA genes use polyadenylation factors to promote efficient transcription termination. Nucleic Acids ResN/A:N/A (2013).
Read more (PubMed: 24097444) »