Anti-Integrin alpha 1 antibody - Cytoplasmic domain (ab78479)

Overview

  • Product nameAnti-Integrin alpha 1 antibody - Cytoplasmic domain
    See all Integrin alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha 1
  • Specificityab78479 is specific to Integrin alpha 1. No cross reactivity to alpha2, alpha3, alpha4, alpha5, alpha6, or alphaV integrin subunits.
  • Tested applicationsSuitable for: ELISA, IP, RIA, WB, ICC, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Goat, Horse, Chicken, Hamster, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Rat Integrin alpha 1 aa 1166-1180 (C terminal). Cytoplasmic domain.
    Sequence:

    KIGFFKRPLKKKMEK

  • Positive control
    • PC12 cells. Rhesus monkey maternal uterine endometrium tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IP Use 5µl for 106 cells.
RIA 1/1000.
WB 1/100 - 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 131 kDa).
ICC Use at an assay dependent dilution.
IHC-P 1/200. Suggested for use on acetone fixed tissue only. This antibody has been reported to react in 4% PFA fixed monkey tissue treated with citrate.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Integrin alpha 1 antibody - Cytoplasmic domain images

  • ICC/IF image of ab78479 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78479, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Integrin alpha 1 antibody - Cytoplasmic domain (ab78479)

This product has been referenced in:
  • Cain EL  et al. Characterization of a human cell line stably over-expressing the candidate oncogene, dual specificity phosphatase 12. PLoS One 6:e18677 (2011). WB ; Human . Read more (PubMed: 21556130) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"