The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at a concentration of 10 µg/ml.
IHC-Fr: Use at a concentration of 20 µg/ml. Acetone fixation recommended.
IP from detergent cell lysates: Use 2µg for 106 cell equivalent.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.
Integrin alpha 1 heterodimerizes with Integrin beta 1 to form a cell-surface receptor for collagen and laminin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis.
Cellular localizationCell membrane; Single-pass type I membrane protein.