PE Anti-Integrin alpha 2 antibody [DX5] (ab25493)
Key features and details
- PE Rat monoclonal [DX5] to Integrin alpha 2
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgM
Overview
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Product name
PE Anti-Integrin alpha 2 antibody [DX5]
See all Integrin alpha 2 primary antibodies -
Description
PE Rat monoclonal [DX5] to Integrin alpha 2 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin alpha 2. NK cells isolated from C57BL/6 mice
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General notes
NK cells cultured in the presence of IL2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. DX5 has not been demonstrated to have activating or blocking activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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Purity
Purified IgM -
Primary antibody notes
NK cells cultured in the presence of IL2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. DX5 has not been demonstrated to have activating or blocking activity. -
Clonality
Monoclonal -
Clone number
DX5 -
Myeloma
Sp2/0 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.2µg for 106 cells.
ab25493 can be used to define functionally distinct subsets of murine NK cells. |
Notes |
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Flow Cyt
Use 0.2µg for 106 cells. ab25493 can be used to define functionally distinct subsets of murine NK cells. |
Target
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Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16398 Mouse
- SwissProt: Q62469 Mouse
- Unigene: 5007 Mouse
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Alternative names
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25493 has been referenced in 1 publication.
- Arase H et al. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol 167:1141-4 (2001). PubMed: 11466327