RabMAb

Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)

Overview

  • Product nameAnti-Integrin alpha 2 antibody [EPR5788]
    See all Integrin alpha 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5788] to Integrin alpha 2
  • Tested applicationsFlow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Human Integrin alpha 2.

  • Positive control
    • T47D, 293T, Human platelet, and A431 lysates; Human colon tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A 40 µl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/1000.
WB 1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 129 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionIntegrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
    • Sequence similaritiesBelongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Integrin alpha 2 antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    Anti-Integrin alpha 2 antibody [EPR5788] images

    • Overlay histogram showing A431 cells stained with ab133557 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133557, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Integrin alpha 2 antibody [EPR5788] (ab133557) at 1/10000 dilution

      Lane 1 : T47D lysate
      Lane 2 : 293T lysate
      Lane 3 : Human platelet lysate
      Lane 4 : A431 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 129 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Integrin alpha 2 with ab133557 at a dilution of 1/250.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Integrin alpha 2 antibody [EPR5788] (ab133557)

    ab133557 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Human Cell (HS 5)
    Specification HS 5
    Permeabilization Yes - Triton X 100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 07 2013

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample Mouse Cell (MC3T3E1)
    Specification MC3T3E1
    Permeabilization Yes - Triton X 100
    Fixative Formaldehyde
    Username

    Mr. Kannan Govindaraj

    Verified customer

    Submitted Aug 06 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution (DAKO)
    Sample Mouse Tissue sections (mammary tumor)
    Specification mammary tumor
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"