The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 105 cells. ab95581 can be used at 0.5 µg/per test. A test is defined as the amount (µg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
ab18479-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Sequence similaritiesBelongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Staining of BALB/c splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 µg of ab95581 (filled histogram) followed by Anti-Armenian Hamster IgG FITC. Cells in the lymphocyte gate were used for analysis.
References for Anti-Integrin alpha 2 antibody [HMa2] (ab95581)
has not yet been referenced specifically in any publications.
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