Anti-Integrin alpha 2b + beta 3 antibody [237] (ab662)

Overview

  • Product nameAnti-Integrin alpha 2b + beta 3 antibody [237]
    See all Integrin alpha 2b + beta 3 primary antibodies
  • Description
    Mouse monoclonal [237] to Integrin alpha 2b + beta 3
  • SpecificityUnder reducing conditions, this antibody will bind against the human platelet membrane glycoproteins IIb and/or IIIa, which have molecular weights of ~130,000 (IIb) and 115,000 (IIIa).
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    109 human platelet suspension. The supernatant fluid from postitive clones was screened via ELISA, the solid phase being coated with washed platelets.

Properties

Applications

Our Abpromise guarantee covers the use of ab662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

This antibody, used at a concentration of 10-20µg/mL will allow visualization of NP 40 solubized normal human platelets

Target

Anti-Integrin alpha 2b + beta 3 antibody [237] images

  • Overlay histogram showing HL60 cells stained with ab662 (red line). The cells were fixed with 4% formaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab662, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Integrin alpha 2b + beta 3 antibody [237] (ab662)

This product has been referenced in:
  • Sobierajska K  et al. Protein disulfide isomerase directly interacts with ß-actin Cys374 and regulates cytoskeleton reorganization. J Biol Chem 289:5758-73 (2014). Human . Read more (PubMed: 24415753) »
  • Soman P & Siedlecki CA Effects of protein solution composition on the time-dependent functional activity of fibrinogen on surfaces. Langmuir 27:10814-9 (2011). Read more (PubMed: 21766803) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The antibody ab662 will detect both alpha 2b and beta 3. I am not sure why this antibody is chosen.

There are other antibodies in catalogue e.g. ab63983, ab134131, ab83961 or ab96794

I hope this i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (Breast cancer cells)
Total protein in input 200 µg
Specification Breast cancer cells
Immuno-precipitation step Protein A/G
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Verified customer

Submitted Apr 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (megakaryocytic)
Loading amount 2e+006 cells
Specification megakaryocytic
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 13 2006

Ab662 is Protein G Affinity Purified. The product has not been tested for cross-reactivity with other glycoproteins, nor is it known whether it has a greater binding affinity for IIb over IIIa or vice-versa. Ab662 has been used under reducing conditio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"