Anti-Integrin alpha 2b + beta 3 antibody [237] (ab662)

Overview

  • Product name
    Anti-Integrin alpha 2b + beta 3 antibody [237]
    See all Integrin alpha 2b + beta 3 primary antibodies
  • Description
    Mouse monoclonal [237] to Integrin alpha 2b + beta 3
  • Specificity
    Under reducing conditions, this antibody will bind against the human platelet membrane glycoproteins IIb and/or IIIa, which have molecular weights of ~130,000 (IIb) and 115,000 (IIIa).
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    109 human platelet suspension. The supernatant fluid from postitive clones was screened via ELISA, the solid phase being coated with washed platelets.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

This antibody, used at a concentration of 10-20µg/mL will allow visualization of NP 40 solubized normal human platelets

Target

Images

  • Overlay histogram showing HL60 cells stained with ab662 (red line). The cells were fixed with 4% formaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab662, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Li X  et al. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells. Sci Rep 7:5081 (2017). WB . Read more (PubMed: 28698547) »
  • Lissat A  et al. IL6 secreted by Ewing sarcoma tumor microenvironment confers anti-apoptotic and cell-disseminating paracrine responses in Ewing sarcoma cells. BMC Cancer 15:552 (2015). IHC . Read more (PubMed: 26215971) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

The antibody ab662 will detect both alpha 2b and beta 3. I am not sure why this antibody is chosen.

There are other antibodies in catalogue e.g. ab63983, ab134131, ab83961 or ab96794

I hope this i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - other (Breast cancer cells)
Total protein in input
200 µg
Specification
Breast cancer cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Apr 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (megakaryocytic)
Loading amount
2e+006 cells
Specification
megakaryocytic
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 13 2006

Ab662 is Protein G Affinity Purified. The product has not been tested for cross-reactivity with other glycoproteins, nor is it known whether it has a greater binding affinity for IIb over IIIa or vice-versa. Ab662 has been used under reducing conditio...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up