Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)

The PO number is XXX.

Thank you so much for your help.

ANSWER:

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.


I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

yes the references do not discuss paraffin sections. I would like to have the refund.

how do I do this?

Thanks.

ANSWER:

Thank you for your reply.


I am sorry to hear that those references were not useful for you. To process the refund, would you please provide your order number or PO? I can then issue the refund as soon as possible.


I look forward to your reply so that I may assist you further.

I stained some slides overnight. I seem to be getting a lot of background, I could increase my blocking step. I will try that and see if it cleans it up. I am staining human tissue.

Is there a paper that specifically uses this antibody in formalin fixed paraffin embedded tissue that I can reference.

Thank you.

ANSWER:

Thank you for you reply.


The
only references we have available are those listed on the datasheet. Unfortunately, I am not able to access them to determine if there is any information regarding the use of this antibody in IHC-P. As I mentioned previously, if these modifications do not improve your results, I am happy to offer a replacement or credit.

We used Heat mediated retrieval at pH 9. The dilution of the antibody was 1/50, 1/100 and 1/200. Room temperature incubation for 30 minutes, with a polymer based detection. And then overnight at4C with the polymer based detection.

This is our routine IHC staining protocol for all clinical work. So this is where I started. There is staining but the pathologist feels that it is non specific. The data sheet lists Breast CA as a control, but what type of breast cancer. The pathologist also said that itshould stain basement membrane in normal epidermis....this is what we could not get good staining with.

Any help would be greatly appreciated.

Thank you.

ANSWER:

Thank you for your reply.


Based on what you have already tested, I am not sure there are many more steps to be optimized. One consideration would be to extend the primary antibody incubation to overnight at 4oC. Longer incubation with less antibody tends to result in a cleaner, more specific signal. I am also happy to send out a new vial of antibody or issue a credit if you would prefer.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

I cannot get this antibody to stain. I am using formalin fixed paraffin embedded tissue. The samples we are staining are skin, and then a TMA block with over thirty different types of tissue in it. I have tried ph6 citrate retrieval, ph9 Tris/EDTA retrieval and protienase K digestion. Any ideas? Thank you.

ANSWER:

Thank you for contacting Abcam regarding ab30483.


I am sorry that you have been experiencing difficulties with this antibody in IHC of paraffin embedded tissue. Heat mediated antigen retrieval using the Tris/EDTA pH 9.0 buffer is the recommended method. I am wondering if you would provide me with some additional details regarding your protocol, such a blocking, antibody dilutions and incubation conditions, and the species and preparation of your tissue.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.