The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Fix with acetone.
Use at an assay dependent concentration.
FunctionIntegrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
Tissue specificityIsoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
Sequence similaritiesBelongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
Post-translational modificationsIsoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
Immunohistochemistry (Frozen sections) analysis of human skin tissue sections labeling Integrin alpha 3 with ab20140 at 1/1000 dilution. An undiluted goat anti-mouse polyclonal alkaline phosphatase conjugate was used as the secondary antibody.
References for Anti-Integrin alpha 3 antibody [F35 177-1] (ab20140)
This product has been referenced in:
Lustosa SA et al. Expression profiling using a cDNA array and immunohistochemistry for the extracellular matrix genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in colorectal carcinoma progression and dissemination. ScientificWorldJournal2014:102541 (2014).
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