Anti-Integrin alpha 3 antibody [F35 177-1] (ab20140)

Overview

  • Product name
    Anti-Integrin alpha 3 antibody [F35 177-1]
    See all Integrin alpha 3 primary antibodies
  • Description
    Mouse monoclonal [F35 177-1] to Integrin alpha 3
  • Tested applications
    Suitable for: IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cell preparation: MTSV1-7 HYE - human breast epithelial cells

  • General notes
    Fusion Partner: NS2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    F35 177-1
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. Fix with acetone.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • Tissue specificity
    Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications
    Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407068 antibody
    • Antigen identified by monoclonal antibody J143 antibody
    • CD49 antigen like family member C antibody
    • CD49 antigen-like family member C antibody
    • CD49c antibody
    • CD49c antigen antibody
    • FLJ34631 antibody
    • FLJ34704 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • Galactoprotein B3 antibody
    • GAP B3 antibody
    • GAPB3 antibody
    • Integrin alpha 3 antibody
    • Integrin alpha-3 light chain antibody
    • Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) antibody
    • ITA3_HUMAN antibody
    • ITGA 3 antibody
    • Itga3 antibody
    • MSK18 antibody
    • VCA 2 antibody
    • VCA2 antibody
    • Very late activation protein 3 receptor alpha 3 subunit antibody
    • VL3A antibody
    • VLA 3 alpha chain antibody
    • VLA 3 subunit alpha antibody
    • VLA-3 subunit alpha antibody
    • VLA3a antibody
    see all

Anti-Integrin alpha 3 antibody [F35 177-1] images

  • Immunohistochemistry (Frozen sections) analysis of human skin tissue sections labeling Integrin alpha 3 with ab20140 at 1/1000 dilution. An undiluted goat anti-mouse polyclonal alkaline phosphatase conjugate was used as the secondary antibody.

References for Anti-Integrin alpha 3 antibody [F35 177-1] (ab20140)

This product has been referenced in:
  • Lustosa SA  et al. Expression profiling using a cDNA array and immunohistochemistry for the extracellular matrix genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in colorectal carcinoma progression and dissemination. ScientificWorldJournal 2014:102541 (2014). IHC ; Human . Read more (PubMed: 24737953) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Blocking step
Labvision Kit as blocking agent for 5 minute(s) · Concentration: 0
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Verified customer

Submitted Nov 30 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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