Overview

  • Product name
    Anti-Integrin alpha 3 antibody [J143]
    See all Integrin alpha 3 primary antibodies
  • Description
    Mouse monoclonal [J143] to Integrin alpha 3
  • Specificity
    This antibody recognizes the alpha 3 subunit of VLA3 (ECMRI - extracellular matrix receptor I, URO1), which has been shown to be related to the chicken fibronectin/laminin receptor. VLA proteins are part of the integrin family of cell adhesion molecules, and the distribution of VLA3 in cells and tissues suggests a possible relationship with cellular adhesion. This antibody detects glycoproteins of 140, 120 and 30kDa.
  • Tested applications
    Suitable for: IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    253J bladder cancer cell line.

  • Positive control
    • Kidney.
  • General notes


    The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.01M PBS
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    The URO panel of antibodies was developed to immuno-anatomically dissect the nephron from glomerulus to bladder urothelium - these antibodies detect various cell surface antigens specific to segments of the renal tubule. Several have been shown to occur as tissue related markers of renal cancers. This antibody reacts predominantly with adherent cell types and is absent from cells growing in suspension, e.g. leukemia, lymphoma, lymphoblastoid, or other hematopoietic cell lines. In tissues, the URO1 antigen is expressed by glomeruli, the basal layer of transitional epithelium and skin, and the basement membrane of glands. All bladder tumors express URO1, as do most kidney tumors and other epithelial tumors (e.g. colon, ovary, lung and breast). Astrocytoma, melanoma, sarcoma, and lymphoma specimens are negative for URO1 expression.
  • Clonality
    Monoclonal
  • Clone number
    J143
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/40. Fix with acetone.
ICC 1/40.

Target

  • Function
    Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • Tissue specificity
    Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications
    Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407068 antibody
    • Antigen identified by monoclonal antibody J143 antibody
    • CD49 antigen like family member C antibody
    • CD49 antigen-like family member C antibody
    • CD49c antibody
    • CD49c antigen antibody
    • FLJ34631 antibody
    • FLJ34704 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • Galactoprotein B3 antibody
    • GAP B3 antibody
    • GAPB3 antibody
    • Integrin alpha 3 antibody
    • Integrin alpha-3 light chain antibody
    • Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) antibody
    • ITA3_HUMAN antibody
    • ITGA 3 antibody
    • Itga3 antibody
    • MSK18 antibody
    • VCA 2 antibody
    • VCA2 antibody
    • Very late activation protein 3 receptor alpha 3 subunit antibody
    • VL3A antibody
    • VLA 3 alpha chain antibody
    • VLA 3 subunit alpha antibody
    • VLA-3 subunit alpha antibody
    • VLA3a antibody
    see all

Anti-Integrin alpha 3 antibody [J143] images

  • ICC/IF image of ab11767 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11767, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Integrin alpha 3 antibody [J143] (ab11767)

This product has been referenced in:
  • Cagnet S  et al. Signaling events mediated by a3ß1 integrin are essential for mammary tumorigenesis. Oncogene N/A:N/A (2013). Read more (PubMed: 24077284) »
  • McKeen Polizzotti L  et al. Novel Image Analysis Approach Quantifies Morphological Characteristics of 3D Breast Culture Acini with Varying Metastatic Potentials. J Biomed Biotechnol 2012:102036 (2012). ICC/IF ; Human . Read more (PubMed: 22665978) »

See all 2 Publications for this product

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