The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
BL of cell cell adhesion: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.