Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] (ab75760)

Overview

  • Product nameAnti-Integrin alpha 4 (phospho S1027) antibody [EPR2343]
    See all Integrin alpha 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2343] to Integrin alpha 4 (phospho S1027)
  • Specificityab75760 only detects Integrin alpha 4 phosphorylated on Serine 1027. The antibody detects the mature chain (180 kDa), the precursor (140 kDa) and the cleaved C terminal (70 kDa).
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Integrin alpha 4 (phospho S1027).
    Database link: P13612

  • Positive control
    • THP1 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 150 kDa.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionIntegrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
    • Sequence similaritiesBelongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • DomainThe SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
    • Post-translational
      modifications
      Phosphorylation on Ser-1027 inhibits PXN binding.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 269C wild type antibody
      • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
      • CD49 antigen like family member D antibody
      • CD49 antigen-like family member D antibody
      • CD49d antibody
      • IA4 antibody
      • Integrin alpha 4 antibody
      • Integrin alpha 4 subunit antibody
      • Integrin alpha IV antibody
      • Integrin alpha-4 antibody
      • Integrin alpha-IV antibody
      • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
      • ITA4_HUMAN antibody
      • ITGA4 antibody
      • MGC90518 antibody
      • OTTHUMP00000205320 antibody
      • Very late activation protein 4 receptor, alpha 4 subunit antibody
      • VLA 4 subunit alpha antibody
      • VLA-4 subunit alpha antibody
      • VLA4 antibody
      see all

    Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] images

    • All lanes : Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] (ab75760) at 1/1000 dilution (purified)

      Lane 1 : rat spleen lysate
      Lane 2 : mouse spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 150 kDa
      Observed band size : 70,150 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] (ab75760) at 1/5000 dilution (purified)

      Lane 1 : untreated Jurkat lysate
      Lane 2 : Jurkat cell lysate treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size : 150 kDa
      Observed band size : 70,140,150 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] (ab75760) at 1/2000 dilution

      Lane 1 : THP1 cell lysates, untreated
      Lane 2 : THP1 cell lysates, with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 150 kDa
      Observed band size : 70,140,180 kDa (why is the actual band size different from the predicted?)

    References for Anti-Integrin alpha 4 (phospho S1027) antibody [EPR2343] (ab75760)

    ab75760 has not yet been referenced specifically in any publications.

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